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Báo cáo y học: " Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:" Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239. | Retrovirology BioMed Central Research Open Access Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239 James B Whitney1 2 3 and Mark A Wainberg 1 2 Address 1McGill University AIDS Centre Lady Davis Institute-Jewish General Hospital Montreal Quebec H3T 1E2 Canada 2Department of Microbiology and Immunology McGill University Montreal Quebec H3A 2B4 Canada and 3Division of Viral Pathogenesis Beth Israel Deaconess Medical Center Harvard Medical School Boston MA 022115 USA Email James BWhitney-jwhitne2@bidmc.harvard.edu Mark AWainberg - mark.wainberg@mcgill.ca Corresponding author Published 21 December 2006 Received 01 November 2006 Accepted 21 December 2006 Retrovirology 2006 3 96 doi 10.1186 1742-4690-3-96 This article is available from http www.retrovirology.cOm content 3 1 96 2006 Whitney and Wainberg licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The 5 untranslated region UTR or leader sequence of simian immunodeficiency virus SIVmac239 is multifunctional and harbors the regulatory elements for viral replication persistence gene translation expression and the packaging and dimerization of viral genomic RNA vRNA . We have constructed a series of deletions in the SIVmac239 leader sequence in order to determine the involvement of this region in both the packaging and dimerization of viral genomic RNA. We also assessed the impact of these deletions upon viral infectiousness replication kinetics and gene expression in cell lines and monkey peripheral blood mononuclear cells PBMC . Results Regions on both sides of the major splice donor SD were found to be necessary for the efficiency and specificity of viral genome packaging. However stem-loop1 is critical for both RNA .