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Báo cáo y học: "Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications. | Comparative Hepatology BioMed Central Research Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka Oryzias latipes methodology and applications Ron C Hardman Seth W Kullman and David E Hinton Open Access Address Duke University Environmental Sciences and Policy Division Nicholas School of the Environment and Earth Sciences LSRC A333 Durham NC USA Email Ron C Hardman - ron.hardman@duke.edu Seth W Kullman - swkull@duke.edu David E Hinton - dhinton@duke.edu Corresponding author Published 6 October 2008 Received 20 March 2007 Accepted 6 October 2008 Comparative Hepatology 2008 7 7 doi 10.1186 1476-5926-7-7 This article is available from http www.comparative-hepatology.com content 7 1 7 2008 Hardman et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background A novel transparent stock of medaka Oryzias latipes STII recessive for all pigments found in chromatophores permits transcutaneous imaging of internal organs and tissues in living individuals. Findings presented describe the development of methodologies for non invasive in vivo investigation in STII medaka and the successful application of these methodologies to in vivo study of hepatobiliary structure function and xenobiotic response in both 2 and 3 dimensions. Results Using brightfield and widefield and confocal fluorescence microscopy coupled with the in vivo application of fluorescent probes structural and functional features of the hepatobiliary system and xenobiotic induced toxicity were imaged at the cellular level with high resolution I pm in living individuals. The findings presented demonstrate I phenotypic response to xenobiotic .