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Báo cáo y học: "A Drosophila protein-interaction map centered on cell-cycle regulators"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: A Drosophila protein-interaction map centered on cell-cycle regulators. | Research Open Access A Drosophila protein-interaction map centered on cell-cycle regulators Clement A Stanyon Guozhen Liu Bernardo A Mangiola Nishi Patel Loic Giot Bing Kuang Huamei Zhang Jinhui Zhong and Russell L Finley Jr Addresses Center for Molecular Medicine Genetics Wayne State University School of Medicine 540 E. Canfield Avenue Detroit MI 48201 USA. CuraGen Corporation 555 Long Warf Drive New Haven CT 06511 USA. Department of Biochemistry and Molecular Biology Wayne State University School of Medicine 540 E. Canfield Avenue Detroit MI 48201 USA. Correspondence Russell L Finley. E-mail rfinley@wayne.edu Published 26 November 2004 Genome Biology 2004 5 R96 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2004A5 12 R96 Received 26 July 2004 Revised 27 October 2004 Accepted 1 November 2004 2004 Stanyon et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Maps depicting binary interactions between proteins can be powerful starting points for understanding biological systems. A proven technology for generating such maps is high-throughput yeast two-hybrid screening. In the most extensive screen to date a Gal4-based two-hybrid system was used recently to detect over 20 000 interactions among Drosophila proteins. Although these data are a valuable resource for insights into protein networks they cover only a fraction of the expected number of interactions. Results To complement the Gal4-based interaction data we used the same set of Drosophila open reading frames to construct arrays for a LexA-based two-hybrid system. We screened the arrays using a novel pooled mating approach initially focusing on proteins related to cell-cycle regulators. .