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Báo cáo y học: "Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites. | Open Access Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites Bernardo J Foth Neng Zhang Sachel Mok Peter R Preiser and Zbynek Bozdech Address School of Biological Sciences Nanyang Technological University Nanyang Drive 637551 Singapore. Correspondence Zbynek Bozdech. Email zbozdech@ntu.edu.sg Published 17 December 2008 Genome Biology 2008 9 R177 doi 10.1186 gb-2008-9- 12-r 177 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2008 9 12 R177 Received 19 September 2008 Revised 1 December 2008 Accepted l7 December 2008 2008 Foth et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Malaria is a one of the most important infectious diseases and is caused by parasitic protozoa of the genus Plasmodium. Previously quantitative characterization of the P. falciparum transcriptome demonstrated that the strictly controlled progression of these parasites through their intra-erythrocytic developmental cycle is accompanied by a continuous cascade of gene expression. Although such analyses have proven immensely useful the correlations between abundance of transcripts and their cognate proteins remain poorly characterized. Results Here we present a quantitative time-course analysis of relative protein abundance for schizont-stage parasites 34 to 46 hours after invasion based on two-dimensional differential gel electrophoresis of protein samples labeled with fluorescent dyes. For this purpose we analyzed parasite samples taken at 4-hour intervals from a tightly synchronized culture and established more than 500 individual protein abundance profiles