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Báo cáo y học: "Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD). | Journal of Immune Based Therapies and Vaccines Original research BioMed Central Open Access Identification of proteases employed by dendritic cells in the processing of protein purified derivative PPD Mansour Mohamadzadeh 1 Hamid Mohamadzadeh2 Melissa Brammer4 Karol Sestak3 and Ronald B Luftig1 Address department of Microbiology Immunology and Parasitology Louisiana State University Health Sciences Center New Orleans LA USA 2Johannes Wolfgang Goethe Medical School Frankfurt Germany 3Tulane National Primate Research Center Science New Orleans Louisiana USA and 4Tulane Medical School New Orleans LA USA Email Mansour Mohamadzadeh - mzadeh@lsuhsc.edu Hamid Mohamadzadeh - mzadeh@lsuhsc.edu Melissa Brammer - mbrammer@tulane.edu Karol Sestak - ksestak@tulane.edu Ronald B Luftig - rlufti@lsuhsc.edu Corresponding author Published 02 August 2004 Received 30 April 2004 Journal of Immune Based Therapies and Vaccines 2004 2 8 doi 10.1186 1476-8518-2-8 Accepted 02 August 2004 This article is available from http www.jibtherapies.cOm content 2 1 8 2004 Mohamadzadeh et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Dendritic cells DC are known to present exogenous protein Ag effectively to T cells. In this study we sought to identify the proteases that DC employ during antigen processing. The murine epidermal-derived DC line Xs52 when pulsed with PPD optimally activated the PPD-reactive Th1 clone LNC.2F1 as well as the Th2 clone LNC.4k1 and this activation was completely blocked by chloroquine pretreatment. These results validate the capacity of XS52 DC to digest PPD into immunogenic peptides inducing antigen specific T cell immune responses. XS52 DC as well as splenic DC and DCs derived from bone marrow degraded standard .