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Báo cáo y học: "Alternative way to test the efficacy of swine FMD vaccines: measurement of pigs median infected dose (PID50) and regulation of live virus challenge dose"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Alternative way to test the efficacy of swine FMD vaccines: measurement of pigs median infected dose (PID50) and regulation of live virus challenge dose | Li et al. Virology Journal 2010 7 215 http www.virologyj.eom content 7 1 215 VIROLOGY JOURNAL SHORT REPORT Open Access Alternative way to test the efficacy of swine FMD vaccines measurement of pigs median infected dose PID50 and regulation of live virus challenge dose Dong Li Zeng-Jun Lu Bao-Xia Xie Pu Sun Ying-Li Chen Yuan-Fang Fu Zai-Xin Liu Abstract Foot-and -mouth disease to pigs is serious recently around the world. Vaccination prevention is still an important policy. OIE specifies 10 000 TCID50 0.2 ml of virulent virus for challenge test in pigs to test the potency of FMD vaccine by intradermal route inoculating the virus in the heel bulbs of one foot or by intramuscular route administering into one site of the neck behind the ear. Convenience and speediness are available in the process of potency test of commercial FMD vaccine. We selected the route of administering into one site of the muscular part of the neck behind the ear because of convenience and speediness. However it was difficult to infect control pigs even up to 100 000TCID50 so we changed the challenged virus from cell-passaged strain to suckling mice-passaged one measured its PID50 pigs median infected dose and defined the virus challenge dose as 1000PID50. Meanwhile we arranged the number of control pigs from two to three for easy evaluation. Findings Foot-and-mouth disease FMD is an extremely contagious viral disease of cloven-hoofed domesticated as well as wild animals and has a great potential for causing severe economic loss. The causal agent FMD virus FMDV is a member of the genus Aphthovirus in the family Picornaviridae and occurs as seven distinct serotypes throughout the world A O C Asia1 and South African Territories SAT 1-3. Vaccination is the most important control and eradication strategy for FMD especially the oil-adjuvant vaccine in developing countries 1-4 . The different processes for preparing vaccines against viral diseases are comprised by a sequence of steps which although .