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Isolation and characterization of bacteria for amylase production under solid state fermentation using damaged wheat as substrate

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A bacterial isolate (I5) along with Bacillus subtilis and Bacillus circulans was evaluated for extracellular amylase production under SmF and SSF conditions. In SmF (Temperature - 28±2°C, pH -7 and inoculum size-106 cfu ml-1 , Time- 96 h), maximum saccharifying activity (SA) was observed with Bacillus subtilis (0.62 IU/ml/min) followed by isolate (I5) (0.58IU/ml/min) and Bacillus circulans (0.46IU/ml/min). Whereas dextrinizing activity (DA) was recorded with Bacillus circulans (1.69U/ml), followed by isolate (I5) (1.65U/ml) and Bacillus subtilis (1.02U/ml). | Isolation and characterization of bacteria for amylase production under solid state fermentation using damaged wheat as substrate

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