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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects. | Int. J. Med. Sci. 2010 7 197 International Journal of Medical Sciences 2010 7 4 197-208 Ivyspring International Publisher. All rights reserved Research Paper Preparation of RGD-modified Long Circulating Liposome Loading Matrine and its in vitro Anti-cancer Effects Xiao-yan Liu1 Li-ming Ruan1 Wei-wei Mao2 Jin-Qiang Wang2 You-qing Shen2 Mei-hua Sui2 1. The First Affiliated Hospital College of Medicine Zhejiang University Hangzhou Zhejiang 310003 China 2. Department of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang 310027 China H Corresponding author Li-ming Ruan The First Affiliated Hospital College of Medicine Zhejiang University 79 Qingchun Road Hangzhou Zhejiang 310003 China. Tel 8613957121201 Email doc1998@yeah.net. Mei-hua Sui Department of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang 310027 China. Tel 8615888847026 email suim@zju.edu.cn Received 2010.05.13 Accepted 2010.06.09 Published 2010.06.14 Abstract Aim To prepare RGD-modified long circulating liposome LCL loading matrine RGD-M-LCL to improve the tumor-targeting and efficacy of matrine. Methods LCL which Was prepared with HSPC cholesterol DspE-PEG2000 and DSPE-PEG-MAL was modified with an RGD motif confirmed by high performance liquid chromatography HPLC . The encapsulation efficiency of RGD-M-LCL was also detected by HPLC. MTT assay was used to examine the effects of RGD-M-LCL on the proliferation of Bcap-37 HT-29 and A375 cells. The percentage of apoptotic cells and morphological changes in Bcap-37 cells treated with RGD-M-LCL were detected by Annexin-V-FITC PI affinity assay and observed under light microscope respectively. Results Spherical or oval single-chamber particles of uniform sizes with little agglutination or adhesion were observed under transmission electronic microscope. The RGD motif was successfully coupled to the DSPE-PEG-MAL on liposomes as confirmed by HPLC. An encapsulation efficiency of 83.1 3 was obtained when the drug-lipid .