Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Evaluation of long-term antinociceptive properties of stabilized hyaluronic acid preparation (NASHA) in an animal model of repetitive joint pain. | Boettger et al. Arthritis Research Therapy 2011 13 R110 http content 13 4 R110 RESEARCH ARTICLE Open Access Evaluation of long-term antinociceptive properties of stabilized hyaluronic acid preparation NASHA in an animal model of repetitive joint pain Michael Karl Boettger1 2 Diana Kummel1 Andrew Harrison3 and Hans-Georg Schaible1 Abstract Introduction Clinical trials provided controversial results on whether the injection of hyaluronan preparations into osteoarthritic joints reduces pain. Problems of clinical studies may be the substantial placebo effects of intraarticular injections different severity and rate of progression of the disease and others. We hypothesize that the use of preclinical pain models may help to clarify whether a certain hyaluronan exerts antinociceptive effects upon intra-articular injection. In the present study we tested in the bradykinin prostaglandin E2 PGE2 model primarily the putative antinociceptive effect of stabilized hyaluronic acid from a non animal source NASHA a stabilized hyaluronic acid based gel for intra-articular treatment of OA. We established a dose-response relationship for NASHA and we compared NASHA to other hyaluronans with different formulations that are in clinical use. Methods To induce transient joint pain episodes bradykinin and PGE2 were repetitively administered intra-articularly and unilaterally into rat knee joints during short anaesthesia. After establishment of the predrug nociceptive responses a single intra-articular injection of saline or NASHA at different concentrations was administered and pain responses to further bradykinin PGE2 injections were monitored up to 56 days after NASHA. Furthermore the obtained effective dose was compared to clinically defined concentrations of Hylan GF20 and sodium hyaluronate. The primary outcome measures were primary mechanical hyperalgesia at the knee joint and pain-induced weight bearing. Results On day 1 after injection all tested hyaluronan .
