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Báo cáo y học: " Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages. | Hoppstădter et al. Respiratory Research 2010 11 124 http respiratory-research.eom content 11 1 124 RESPIRATORY RESEARCH RESEARCH Open Access Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages 1 1 1 T- i- 2 3 4 Jessica Hoppstadter Britta Diesel Robert Zarbock Tanja Breinig Dominik Monz Marcus Koch 25 3 6 7 1 Andreas Meyerhans Ludwig Gortner Claus-Michael Lehr Hanno Huwer Alexandra K Kiemer Abstract Background Investigations on pulmonary macrophages MF mostly focus on alveolar MF AM as a well-defined cell population. Characteristics of MF in the interstitium referred to as lung interstitial MF IM are rather ill-defined. In this study we therefore aimed to elucidate differences between AM and IM obtained from human lung tissue. Methods Human AM and IM were isolated from human non-tumor lung tissue from patients undergoing lung resection. Cell morphology was visualized using either light electron or confocal microscopy. Phagocytic activity was analyzed by flow cytometry as well as confocal microscopy. Surface marker expression was measured by flow cytometry. Toll-like receptor TLR expression patterns as well as cytokine expression upon TLR4 or TLR9 stimulation were assessed by real time RT-PCR and cytokine protein production was measured using a fluorescent bead-based immunoassay. Results IM were found to be smaller and morphologically more heterogeneous than AM whereas phagocytic activity was similar in both cell types. HLA-DR expression was markedly higher in IM compared to AM. Although analysis of TLR expression profiles revealed no differences between the two cell populations AM and IM clearly varied in cell reaction upon activation. Both MF populations were markedly activated by LPS as well as DNA isolated from attenuated mycobacterial strains M. bovis H37Ra and BCG . Whereas AM expressed higher amounts of inflammatory cytokines upon activation IM were more efficient in producing .