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Báo cáo y học: "Reduced immunomodulation potential of bone marrow-derived mesenchymal stem cells induced CCR4+CCR6+ Th/Treg cell subset imbalance in ankylosing spondylitis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Reduced immunomodulation potential of bone marrow-derived mesenchymal stem cells induced CCR4+CCR6+ Th/Treg cell subset imbalance in ankylosing spondylitis. | Wu et al. Arthritis Research Therapy 2011 13 R29 http arthritis-research.eom content 13 1 R29 RESEARCH ARTICLE Open Access Reduced immunomodulation potential of bone marrow-derived mesenchymal stem cells induced CCR4 CCR6 Th Treg cell subset imbalance in ankylosing spondylitis 1Ỷ 2Ỷ 2 2 3 -1-2 2 2 Yanfeng Wu Mingliang Ren Rui Yang Xinjun Liang Yuanchen Ma Yong Tang Lin Huang Jichao Ye Keng Chen 2 Peng Wang2 Huiyong Shen2 Abstract Introduction Ankylosing spondylitis AS is a chronic autoimmune disease and the precise pathogenesis is largely unknown at present. Bone marrow-derived mesenchymal stem cells BMSCs with immunosuppressive and antiinflammatory potential and Th17 Treg cells with a reciprocal relationship regulated by BMSCs have been reported to be involved in some autoimmune disorders. Here we studied the biological and immunological characteristics of BMSCs the frequency and phenotype of CCR4 CCR6 Th Treg cells and their interaction in vitro in AS. Methods The biological and immunomodulation characteristics of BMSCs were examined by induced multipledifferentiation and two-way mixed peripheral blood mononuclear cell PBMC reactions or after stimulation with phytohemagglutinin respectively. The interactions of BMSCs and PBMCs were detected with a direct-contact coculturing system. CCR4 CCR6 Th Treg cells and surface markers of BMSCs were assayed using flow cytometry. Results The AS-BMSCs at active stage showed normal proliferation cell viability surface markers and multiple differentiation characteristics but significantly reduced immunomodulation potential decreased 68 14 the frequencies of Treg and Fox-P3 cells in AS-PBMCs decreased while CCR4 CCR6 Th cells increased compared with healthy donors. Moreover the AS-BMSCs induced imbalance in the ratio of CCR4 CCR6 Th Treg cells by reducing Treg PBMCs and increasing CCR4 CCR6 Th PBMCs and also reduced Fox-P3 cells when co-cultured with PBMCs. Correlation analysis showed that the immunomodulation potential of BMSCs