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Báo cáo y học: "Identification of target antigens of antiendothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of target antigens of antiendothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach. | Régent et al. Arthritis Research Therapy 2011 13 R107 http arthritis-research.eom content 13 3 R107 RESEARCH ARTICLE Open Access Identification of target antigens of anti-endothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis a proteomic approach A I z-w I r D A z t 1 2 3 I_I s s Z-J I 1 L .1 2 I I_I I 1 2 41 p. r I r I A Z t s r z l 5tK hi ii I V V I p. r1 2 l I I s s I i I s ws nr1 2 Alexis Regent Hdnddi DID Kim H Ly Cnristidn Agdrd Mdtnieu C Idmby Nicolds Idmds Babette Weksler6 Christidn Federici1 2 Cedric Broussdrd7 Loic Guillevin2 3 dnd Luc Mouthon1 2 3 Abstract Introduction Immunologicdl studies of gidnt cell drteritis GCA suggest thdt d triggering dntigen of unknown ndture could generate d specific immune response. We thus decided to detect autoantibodies directed dgdinst endothelidl cells ECs dnd vdsculdr smooth muscle cells VSMCs in the serum of GCA pdtients dnd to identify their tdrget dntigens. Methods Serd from 15 GCA pdtients were tested in 5 pools of 3 pdtients serd dnd compdred to d serd pool from 12 hedlthy controls HCs . Serum immunoglobulin G IgG redctivity wds dndlysed by 2-D electrophoresis dnd immunoblotting with dntigens from humdn umbilicdl vein ECs HUVECs dnd mdmmdry drtery VSMCs. Tdrget dntigens were identified by mdss spectrometry. Results Serum IgG from GCA pdtients recognised 162 3 medn SD dnd 100 17 medn SD protein spots from HUVECs dnd VSMCs respectively dnd thdt from HCs recognised 79 dnd 94 protein spots respectively. In totdl 30 spots from HUVECs dnd 19 from VSMCs were recognised by dt ledst two-thirds dnd three-fifths respectively of the pools of serd from GCA pdtients dnd not by serd from HCs. Among identified proteins we found vinculin ldmin A C voltdge-dependent dnion-selective chdnnel protein 2 dnnexin V dnd other proteins involved in cell energy metdbolism dnd key celluldr pdthwdys. Ingenuity pdthwdy dndlysis revedled thdt most identified tdrget dntigens interacted with growth .