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Báo cáo y học: "Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence. | Respiratory Research BioMed Central Research Open Access Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence Dianne M Kube David Fletcher and Pamela B Davis Address Department of Pediatrics Case Western Reserve University School of Medicine BRB 8th floor 2109 Adelbert Rd. Cleveland OH 44106 USA Email Dianne M Kube - dmkube@adelphia.net David Fletcher - david.fletcher@case.edu Pamela B Davis - pamela.davis@case.edu Corresponding author Published II July 2005 Received 19 April 2005 Respiratory Research 2005 6 69 doi 10.1186 1465-9921 -6-69 Accepted 11 July 2005 This article is available from http respiratory-research.cOm content 6 1 69 2005 Kube et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract In many model systems cystic fibrosis CF phenotype airway epithelial cells in culture respond to P. aeruginosa with greater interleukin IL -8 and IL-6 secretion than matched controls. In order to test whether this excess inflammatory response results from the reported increased adherence of P. aeruginosa to the CF cells we compared the inflammatory response of matched pairs of CF and non CF airway epithelial cell lines to the binding of GFP-PAO1 a strain of pseudomonas labeled with green fluorescent protein. There was no clear relation between GFP-PAO1 binding and cytokine production in response to PAO1. Treatment with exogenous aGM1 resulted in greater GFP-PAO1 binding to the normal phenotype compared to CF phenotype cells but cytokine production remained greater from the CF cell lines. When cells were treated with neuraminidase PAO1 adherence was equalized between CF and nonCF phenotype cell lines but IL-8 production in response to inflammatory stimuli was still greater in CF .