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Báo cáo y học: "Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation. | Kim et al. Critical Care 2010 14 R61 http ccforum.eom content 14 2 R61 c CRITICAL CARE RESEARCH Open Access Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation Oh Yoen Kim 1 Antoine Monsel2 Michèle Bertrand2 Pierre Coriat2 Jean-Marc Cavaillon 1 and Minou Adib-Conquy1 Abstract Introduction Decreased expression of human leukocyte antigen class II HLA-DR on monocytes is a hallmark of altered immune status in patients with a systemic inflammatory response syndrome SIRS . So far the analyses were mainly performed without taking into account monocytes subpopulations. Methods We studied this modification on CD14HIGH and CD14LOW monocytes of 20 SIRS patients undergoing abdominal aortic surgery AAS 20 patients undergoing carotid artery surgery CAS and 9 healthy controls and we investigated mediators and intracellular molecules that may be involved in this process. Results HLA-DR on CD14HIGH monocytes started to decrease during surgery after blood reperfusion and was further reduced post-surgery. In contrast HLA-DR expression on CD14LOW cells only decreased after surgery and to a lesser extent than on CD14HIGH monocytes. Negative correlations were found between the reduction of HLA-DR expression and the change in cortisol levels for both subpopulations whereas a negative correlation between interleukin-10 IL-10 levels and HLA-DR modulation was only observed for CD14HIGH cells. In accordance with these ex vivo results HLA-DR on CD14HIGH and CD14LOW monocytes of healthy donors was reduced following incubation with hydrocortisone whereas IL-10 only acted on CD14HIGH subpopulation. Furthermore flow cytometry revealed that the expression of IL-10 receptor was higher on CD14HIGH versus CD14LOW monocytes. In addition hydrocortisone and to a lesser extent IL-10 reversed the up-regulation of HLA-DR induced by bacterial products. Finally membrane-associated RING-CH-1 protein MARCH1 mRNA a negative regulator of MHC class II was up-regulated in