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Báo cáo y học: "Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors. | Research Open Access Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors Gustavo A de Souza Lyris MF de Godoy and Matthias Mann Addresses Center for Experimental BioInformatics CEBI Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej DK-5230 Odense M Denmark. Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz D-82152 Martinsried Germany. Correspondence Matthias Mann. Email mmann@biochem.mpg.de Published 10 August 2006 Genome Biology 2006 7 R72 doi 10.1 l86 gb-2006-7-8-r72 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2006 7 8 R72 Received 12 April 2006 Revised 30 May 2006 Accepted 10 August 2006 2006 de Souza et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The tear film is a thin layer of fluid that covers the ocular surface and is involved in lubrication and protection of the eye. Little is known about the protein composition of tear fluid but its deregulation is associated with disease states such as diabetic dry eyes. This makes this body fluid an interesting candidate for in-depth proteomic analysis. Results In this study we employ state-of-the-art mass spectrometric identification using both a hybrid linear ion trap-Fourier transform LTQ-FT and a linear ion trap-Orbitrap LTQ-Orbitrap mass spectrometer and high confidence identification by two consecutive stages of peptide fragmentation MS MS MS or MS3 to characterize the protein content of the tear fluid. Low microliter amounts of tear fluid samples were either pre-fractionated with one-dimensional SDS-PAGE and digested in situ