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báo cáo hóa học:" The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài :The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome | Virology Journal BioMed Central Research Open Access The 3 sequences required for incorporation of an engineered ssRNA into the Reovirus genome Michael R Roner and Joanne Roehr Address Department of Biology The University of Texas at Arlington Arlington TX 76019 USA Email Michael R Roner - roner@uta.edu Joanne Roehr - roehr@uta.edu Corresponding author Published 03 January 2006 Received 05 October 2005 Accepted 03 January 2006 Virology Journal 2006 3 1 doi 10.1186 1743-422X-3-1 This article is available from http www.virologyj.com content 3 1 1 2006 Roner and Roehr licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Understanding how an organism replicates and assembles a multi-segmented genome with fidelity previously measured at 100 presents a model system for exploring questions involving genome assortment and RNA protein interactions in general. The virus family Reoviridae containing nine genera and more than 200 members are unique in that they possess a segmented double-stranded ds RNA genome. Using reovirus as a model member of this family we have developed the only functional reverse genetics system for a member of this family with ten or more genome segments. Using this system we have previously identified the flanking 5 sequences required by an engineered s2 ssRNA for efficient incorporation into the genome of reovirus. The minimum 5 sequence retains 96 nucleotides and contains a predicted sequence structure element. Within these 96 nucleotides we have identified three nucleotides A-U-U at positions 79-81 that are essential for the incorporation of in vitro generated ssRNAs into new reovirus progeny viral particles. The work presented here builds on these findings and presents the results of an .