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Báo cáo khoa học: Secretion of proteases in serglycin transfected Madin–Darby canine kidney cells

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Madin–Darby canine kidney (MDCK) cells, which do not normally express the proteoglycan (PG) serglycin, were stably transfected with cDNA for human serglycin fused to a polyhistidine tag (His-tag). Clones with differ-ent levels of serglycin mRNA expression were generated. One clone with lower and one with higher serglycin mRNA expression were selected for this study.

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