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Báo cáo khoa học: "Commercially Available Antibodies to Human Tumour Necrosis Factor-α Tested for CrossReactivity with Ovine and Bovine Tumour Necrosis Factor-α using Flow Cytometric Assay"

Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: Commercially Available Antibodies to Human Tumour Necrosis Factor-α Tested for CrossReactivity with Ovine and Bovine Tumour Necrosis Factor-α using Flow Cytometric Assays. | Acta vet. scand. 2004 45 99-107. Commercially Available Antibodies to Human Tumour Necrosis Factor-a Tested for Cross- Reactivity with Ovine and Bovine Tumour Necrosis Factor-a using Flow Cytometric Assays By J. Dernfalk1 K. Persson Waller2 3 and A. Johannisson1 department of Anatomy and Physiology Swedish University of Agricultural Sciences department of Ruminant and Porcine Diseases National Veterinary Institute and department of Obstetrics and Gynaecology Swedish University of Agricultural Sciences Uppsala Sweden. Dernfalk J Persson Waller K Johannisson A Commercially available antibodies to human tumour necrosis factor-a tested for cross-reactivity with ovine and bovine tumour necrosis factor-a using flow cytometric assays. Acta vet. scand. 2004 45 99-107. - A thorough understanding of the immune system including the role of different cytokines during inflammatory diseases in ruminants could lead to the development of new diagnostic methods and treatments. Tumour necrosis factor-a TNF-a is an important cytokine in the onset of the inflammatory responses. Unfortunately the number of studies on cytokines like TNF-a in ruminants is limited due to a lack of species-specific reagents. As cytokines have remained rather conserved during evolution cross-reactivity between animal species may occur. Therefore the aim of the present study was to investigate 5 commercially available antibodies against human TNF-a for their ability to cross-react with ovine and or bovine TNF-a using a bead-based flow cytometric method. Two of the antibody clones Mab 11 and 6401.1111 showed cross reactivity with ovine recombinant TNF-a in concentrations above 2.5ng ml. However none of the antibodies detected TNF-a in bovine milk or serum containing known concentrations of bovine TNF-a as earlier determined with ELISA. The results could be due to inability of the antibodies to cross-react between species but quenching of the signal by matrix proteins might also have lowered the response. .