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Báo cáo y học: "Endothelial Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Endothelial Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients. | Bordes et al. Critical Care 2011 15 412 http ccforum.eom content 15 2 412 CRITICAL CARE LETTER Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients Julien Bordes 1 2 Tiphaine Gaillard3 Jerome Maslin3 Pierre Esnault 2 Philippe Goutorbe2 and Patrick Brisou2 Cytomegalovirus CMV reactivation has been widely documented in the past 10 years in critically ill patients 1 . Conversely few data are available on burn patients despite experimental studies showing that these patients are predisposed to herpes virus infections 2 . To our knowledge only two studies reported the incidence of CMV infection in burn patients using a modern technique such as PCR which has become the gold standard 3 4 . These two studies demonstrated a high rate of CMV reactivation 55 and 71 respectively. Moreover CMV reactivation in burn patients has been proven to be intense. Indeed in the study of Bordes and colleagues 4 67 of patients who reactivated CMV experienced viremia greater than 1 000 copies ml and 33 viremia greater than 10 000 copies ml. These results may reflect the severe immunosuppression that characterizes thermally injured patients. Consequently severe burn patients could be considered as a model for CMV reactivation in critically ill patients. However the precise kinetics of CMV DNA load in these patients is still poorly documented. That is why we would like to briefly present data from longitudinal monitoring of CMV infection by real-time PCR RT-PCR in four severe burn patients during their ICU stay Figure 1 . All the patients were CMV IgG seropositive on admission. They were monitored for CMV reactivation once to twice a week. Detection of CMV DNA in blood samples was performed by quantitative RT-PCR on whole blood. The patients characteristics are described in Table 1. Patient 2 presented a CMV-associated hemophagocytic syndrome and was treated by ganciclovir for a duration of 21 days. DNAemia became undetectable in patients 3 and 4 .