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Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts. | Open Access Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts Joshua Z Levin Michael F Berger Xian Adiconis Peter Rogov Alexandre Melnikov Timothy Fennell Chad Nusbaum Levi A Garraway and Andreas Gnirke Addresses Genome Sequencing and Analysis Program Broad Institute of MIT and Harvard 320 Charles Street Cambridge MA 02141 USA. Cancer Program Broad Institute of MIT and Harvard 5 Cambridge Center Cambridge MA 02142 USA. Sequencing Platform Broad Institute of MIT and Harvard 320 Charles Street Cambridge MA 02141 USA. Department of Medical Oncology and Center for Cancer Genome Discovery Dana-Farber Cancer Institute Harvard Medical School 44 Binney Street Boston MA 02115 USA. Correspondence Joshua Z Levin. Email jlevin@broadinstitute.org Published 16 October 2009 Genome Biology 2009 10 R115 doi 10.1186 gb-2009-10-10-r115 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 10 10 R115 Received 20 August 2009 Revised 23 September 2009 Accepted 16 October 2009 2009 Levin licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes this method showed high selectivity improved mutation detection enabling discovery of novel chimeric transcripts and provided RNA expression data. Thus targeted RNA-Seq produces an enhanced view of the molecular state of a set of high interest genes. Background In recent years a technologic revolution has .