Đánh giá tính an toàn và hiệu lực của vaccine PRRS nhược độc chủng BG895 trên heo thí nghiệm

Bài viết Đánh giá tính an toàn và hiệu lực của vaccine PRRS nhược độc chủng BG895 trên heo thí nghiệm trình bày các kết quả đánh giá tính an toàn và hiệu lực vaccine chủng BG895 đối với PRRSV trên heo nuôi thực nghiệm. | VNU Journal of Science Natural Sciences and Technology Vol No 20 1-10 Original Article The Safety and Efficacy Evaluation of PRRS Attenuated Vaccine BG895 Strain in Experimental Pigs Bui Anh Thy1 2 Nguyen Tang Truong1 Kim Van Phuc1 Nguyen Thien Thu1 Nguyen Van Dung1 Tran Xuan Hanh1 Tran Linh Thuoc2 1 NAVETCO National Veterinary Joint Stock Company 29A Nguyen Dinh Chieu Ho Chi Minh City Vietnam 2 University of Science VNU-HCM 227 Nguyen Van Cu Ho Chi Minh City Vietnam Received 10 August 2021 Revised 25 September 2021 Accepted 30 September 2021 Abstract From the PRRSV virulent strain BG8 isolated from a PRRSV-infected pig using serial passage method in MARC-145 cell line we have successfully obtained an attenuated strain in 95 th passage named as BG895 with high potential to be a vaccine candidate. In this study we present the results of the safety and efficacy evaluation of BG895 against PRRSV in experimental pigs. Trial results of vaccine formula using strain BG895 have very high safety when inoculating 5 doses animal and 10 doses animal. Evaluation of immune response by ELISA method showed that anti-PRRSV antibodies were detected in the serum of all inoculated pigs in vaccine batches with the lowest S P index of from 14 days post inoculation and the highest S P was from 28 days post inoculation. The IPMA method showed that the antibody titer of the vaccine reached 160 in 100 of pigs from 21 days post inoculation and reached 640 in 100 of pigs from 28 days post inoculation indicating that the vaccine was effective at protecting 100 of pigs from 28 days post inoculation. The protective effect of the vaccine was evaluated by the virulent challenge from 28 days post inoculation with 1 dose animal compared with the control group. The results showed that compared with all pigs in the control group with typical clinical manifestations of Blue-ear disease all inoculated pigs had normal body temperature and weight gain besides the S P index increased .

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