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báo cáo khoa học: "Dysregulation of miR-15a and miR-214 in human pancreatic cancer"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Dysregulation of miR-15a and miR-214 in human pancreatic cancer | Zhang et al. Journal of Hematology Oncology 2010 3 46 http www.jhoonline.Org content 3 1 46 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access Dysregulation of miR-15a and miR-214 in human pancreatic cancer Xing J ZhangH Hua Ye2t Cheng W Zeng1 Bo He2 Hua Zhang1 Yue Q Chen1 Abstract Background Recent reports indicate that microRNAs miRNAs play a critical role in malignancies. However the role that miRNAs play in pancreatic cancer remains to be determined. The purpose of this study was to investigate aberrantly expressed miRNAs in pancreatic cancer tissues and demonstrate their roles in disease progression. Results We detected the expression patterns of miRNAs in 10 pancreatic cancer tissues and their adjacent benign tissues by quantitative real time-PCR qRT-PCR and found that miR-15a and miR-214 were dysregulated in the tumor samples. This is the first time that miR-214 has been identified as aberrantly expressed in pancreatic cancer. In vitro experiments showed that overexpression of miR-15a inhibited the viability of pancreatic cancer cells whereas overexpression of miR-214 decreased the sensitivity of the cells to gemcitabine GEM . Furthermore we identified WNT3A and FGF7 as potential targets of miR-15a and ING4 as a target of miR-214. Conclusions Aberrant expression of miRNAs such as miR-15a and miR-214 results in different cellular effects in pancreatic cancer. Downregulation of miR-15a might contribute to proliferation of pancreatic cancer cells whereas upregulation of miR-214 in pancreatic cancer specimens might be related to the poor response of pancreatic cancer cells to chemotherapy. MiR-15a directly targets multiple genes relevant in pancreatic cancer suggesting that it may serve as a novel therapeutic target for treatment of the disease. Background Pancreatic cancer is a disease with a high rate of mortality. It is generally diagnosed at an advanced stage at which point no successful therapies are available. Pancreatic cancer is characterized by the .