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báo cáo khoa học: "Combinatorial peptidomics: a generic approach for protein expression profiling"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Combinatorial peptidomics: a generic approach for protein expression profiling | Journal of Nanobiotechnology BioMed Central Research Open Access Combinatorial peptidomics a generic approach for protein expression profiling Mikhail Soloviev Richard Barry Elaine Scrivener and Jonathan Terrett Address Oxford GlycoSciences UK Ltd Abingdon Oxon OX14 3YS United Kingdom Email Mikhail Soloviev - Mikhail.Soloviev@ogs.co.uk Richard Barry - Richard.Barry@ogs.co.uk Elaine Scrivener - Elaine.Scrivener@ogs.co.uk Jonathan Terrett - Jon.Terrett@ogs.co.uk Corresponding author Published 03 July 2003 Received 28 May 2003 Accepted 03 July 2003 Journal of Nanobiotechnology 2003 1 4 This article is available from http www.jnanobiotechnology.cOm content 1 1 4 2003 Soloviev et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Traditional approaches to protein profiling were built around the concept of investigating one protein at a time and have long since reached their limits of throughput. Here we present a completely new approach for comprehensive compositional analysis of complex protein mixtures capable of overcoming the deficiencies of current proteomics techniques. The Combinatorial methodology utilises the peptidomics approach in which protein samples are proteolytically digested using one or a combination of proteases prior to any assay being carried out. The second fundamental principle is the combinatorial depletion of the crude protein digest i.e. of the peptide pool by chemical crosslinking through amino acid side chains. Our approach relies on the chemical reactivities of the amino acids and therefore the amino acid content of the peptides i.e. their information content rather than their physical properties. Combinatorial peptidomics does not use affinity reagents and relies on neither chromatography nor electrophoretic separation techniques. It is the first generic