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Báo cáo khoa hoc:" Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems | Journal of Negative Results in BioMedicine BioMed Central Research Open Access Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems Tiina Laitala-Leinonen Address Institute of Biomedicine Department of Anatomy University of Turku Turku Finland Email Tiina Laitala-Leinonen - tilale@utu.fi Corresponding author Published 29 August 2005 Received 18 January 2005 Journal of Negative Results in BioMedicine 2005 4 5 doi 10.1186 1477-5751-4- Accepted 29 August 2005 5 This article is available from http www.jnrbm.com content 4 1 5 2005 Laitala-Leinonen licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Bone-resorbing osteoclasts are multinucleated cells that are formed via fusion of their hematopoietic stem cells. Many of the details of osteoclast formation activation and motility remain unsolved. Therefore there is an interest among bone biologists to transfect the terminally differentiated osteoclasts and follow their responses to the transgenes in vitro. Severe difficulties in transfecting the large adherent osteoclasts have been encountered however making the use of modern cell biology tools in osteoclast research challenging. Transfection of mature osteoclasts by non-viral gene transfer systems has not been reported. Results We have systematically screened the usefulness of several commercial DNA transfection systems in human osteoclasts and their mononuclear precursor cell cultures and compared transfection efficacy to adenoviral DNA transfection. None of the liposome-based or endosome disruption-inducing systems could induce EGFP-actin expression in terminally differentiated osteoclasts. Instead a massive cell death by apoptosis was found with all concentrations and