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báo cáo khoa học: "Apple skin patterning is associated with differential expression of MYB10"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Apple skin patterning is associated with differential expression of MYB10 | BMC Plant Biology Apple skin patterning is associated with differential expression of MYB10 Telias et al. Telias et al. BMC Plant Biology 2011 11 93 http www.biomedcentral.com 1471-2229 11 93 20 May 2011 BioMed Central Telias et al. BMC Plant Biology 2011 11 93 http www.biomedcentral.com 1471-2229 11 93 BMC Plant Biology RESEARCH ARTICLE Open Access Apple skin patterning is associated with differential expression of MYB10 1 2 3 3 2 2 4 Adriana Telias Kui Lin-Wang David E Stevenson Janine M Cooney Roger P Hellens Andrew C Allan Emily E Hoover5 and James M Bradeen6 Abstract Background Some apple Malus X domestica Borkh. varieties have attractive striping patterns a quality attribute that is important for determining apple fruit market acceptance. Most apple cultivars e.g. Royal Gala produce fruit with a defined fruit pigment pattern but in the case of Honeycrisp apple trees can produce fruits of two different kinds striped and blushed. The causes of this phenomenon are unknown. Results Here we show that striped areas of Honeycrisp and Royal Gala are due to sectorial increases in anthocyanin concentration. Transcript levels of the major biosynthetic genes and MYB10 a transcription factor that upregulates apple anthocyanin production correlated with increased anthocyanin concentration in stripes. However nucleotide changes in the promoter and coding sequence of MYB10 do not correlate with skin pattern in Honeycrisp and other cultivars differing in peel pigmentation patterns. A survey of methylation levels throughout the coding region of MYB10 and a 2.5 Kb region 5 of the ATG translation start site indicated that an area 900 bp long starting 1400 bp upstream of the translation start site is highly methylated. Cytosine methylation was present in all three contexts with higher methylation levels observed for CHH and CHG where H is A C or T than for CG. Comparisons of methylation levels of the MYB10 promoter in Honeycrisp red and green stripes indicated that they correlate