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báo cáo khoa học: " The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathway"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathway | Faria et al. BMC Plant Biology 2011 11 129 http www.biomedcentral.com 1471 -2229 11 129 BMC Plant Biology RESEARCH ARTICLE Open Access The NAC domain-containing protein GmNAC6 is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathway 1 1.2 1 1.2 1 Jerusa AQA Faria Pedro AB Reis Marco TB Reis Gustavo L Rosado Guilherme L Pinheiro Giselle C Mendes1 2 and Elizabeth PB Fontes1 2 Abstract Background The endoplasmic reticulum ER is a major signaling organelle which integrates a variety of responses against physiological stresses. In plants one such stress-integrating response is the N-rich protein NRP -mediated cell death signaling pathway which is synergistically activated by combined ER stress and osmotic stress signals. Despite the potential of this integrated signaling to protect plant cells against different stress conditions mechanistic knowledge of the pathway is lacking and downstream components have yet to be identified. Results In the present investigation we discovered an NAC domain-containing protein from soybean GmNAC6 Glycine max NAC6 to be a downstream component of the integrated pathway. Similar to NRP-A and NRP-B GmNAC6 is induced by ER stress and osmotic stress individually but requires both signals for full activation. Transient expression of GmNAC6 promoted cell death and hypersensitive-like responses in planta. GmNAC6 and NRPs also share overlapping responses to biotic signals but the induction of NRPs peaked before the increased accumulation of GmNAC6 transcripts. Consistent with the delayed kinetics of GmNAC6 induction increased levels of NRP-A and NRP-B transcripts induced promoter activation and the expression of the GmNAC6 gene. Conclusions Collectively our results biochemically link GmNAC6 to the ER stress- and osmotic stress-integrating cell death response and show that GmNAC6 may act downstream of the NRPs. Keywords GmNAC6 Cell death ER stress osmotic stress NRPs N-rich proteins .