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báo cáo khoa học: "Transcriptomics of shading-induced and NAAinduced abscission in apple (Malus domestica) reveals a shared pathway involving reduced photosynthesis, alterations in carbohydrate transport and signaling and hormone crosstalk"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptomics of shading-induced and NAAinduced abscission in apple (Malus domestica) reveals a shared pathway involving reduced photosynthesis, alterations in carbohydrate transport and signaling and hormone crosstalk | Zhu et al. BMC Plant Biology 2011 11 138 http www.biomedcentral.com 1471-2229 11 138 BMC Plant Biology RESEARCH ARTICLE Open Access Transcriptomics of shading-induced and NAA-induced abscission in apple Malus domestica reveals a shared pathway involving reduced photosynthesis alterations in carbohydrate transport and signaling and hormone crosstalk 13 2 3 2 rx X 1.3 13 Hong Zhu Chris D Dardick Eric P Beers Ann M Callanhan Rui Xia and Rongcai Yuan Abstract Background Naphthaleneacetic acid NAA a synthetic auxin analogue is widely used as an effective thinner in apple orchards. When applied shortly after fruit set some fruit abscise leading to improved fruit size and quality. However the thinning results of NAA are inconsistent and difficult to predict sometimes leading to excess fruit drop or insufficient thinning which are costly to growers. This unpredictability reflects our incomplete understanding of the mode of action of NAA in promoting fruit abscission. Results Here we compared NAA-induced fruit drop with that caused by shading via gene expression profiling performed on the fruit abscission zone FAZ sampled 1 3 and 5 d after treatment. More than 700 genes with significant changes in transcript abundance were identified from NAA-treated FAZ. Combining results from both treatments we found that genes associated with photosynthesis cell cycle and membrane cellular trafficking were downregulated. On the other hand there was up-regulation of genes related to ABA ethylene biosynthesis and signaling cell wall degradation and programmed cell death. While the differentially expressed gene sets for NAA and shading treatments shared only 25 identity NAA and shading showed substantial similarity with respect to the classes of genes identified. Specifically photosynthesis carbon utilization ABA and ethylene pathways were affected in both NAA- and shading-induced young fruit abscission. Moreover we found that NAA similar to shading directly interfered with leaf .