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báo cáo khoa học: " The salt-responsive transcriptome of chickpea roots and nodules via deepSuperSAGE"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The salt-responsive transcriptome of chickpea roots and nodules via deepSuperSAGE | Molina et al. BMC Plant Biology 2011 11 31 http www.biomedcentral.com 1471-2229 11 31 BMC Plant Biology RESEARCH ARTICLE Open Access The salt-responsive transcriptome of chickpea roots and nodules via deepSuperSAGE 16 3 14 5 1 2 Carlos Molina 1 Mainassara Zaman-Allah Faheema Khan 1 Nadia Fatnassi Ralf Horres Bjorn Rotter Diana Steinhauer2 Laurie Amenc3 Jean-Jacques Drevon3 Peter Winter2 Gunter Kahl1 Abstract Background The combination of high-throughput transcript profiling and next-generation sequencing technologies is a prerequisite for genome-wide comprehensive transcriptome analysis. Our recent innovation of deepSuperSAGE is based on an advanced SuperSAGE protocol and its combination with massively parallel pyrosequencing on Roche s 454 sequencing platform. As a demonstration of the power of this combination we have chosen the salt stress transcriptomes of roots and nodules of the third most important legume crop chickpea Cicer arietinum L. . While our report is more technology-oriented it nevertheless addresses a major world-wide problem for crops generally high salinity. Together with low temperatures and water stress high salinity is responsible for crop losses of millions of tons of various legume and other crops. Continuously deteriorating environmental conditions will combine with salinity stress to further compromise crop yields. As a good example for such stress-exposed crop plants we started to characterize salt stress responses of chickpeas on the transcriptome level. Results We used deepSuperSAGE to detect early global transcriptome changes in salt-stressed chickpea. The salt stress responses of 86 919 transcripts representing 17 918 unique 26 bp deepSuperSAGE tags UniTags from roots of the salt-tolerant variety INRAT-93 two hours after treatment with 25 mM NaCl were characterized. Additionally the expression of 57 281 transcripts representing 13 115 UniTags was monitored in nodules of the same plants. From a total of 144 200 analyzed 26 bp tags in .