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báo cáo khoa học: " Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea mays L.)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea mays L.) | Brenner et al. BMC Plant Biology 2010 10 27 http www.biomedcentral.com 1471-2229 10 27 BMC Plant Biology RESEARCH ARTICLE Open Access Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize Zea mays L. 12 1 3 2 4 Everton A Brenner Imad Zein Yongsheng Chen Jeppe R Andersen Gerhard Wenzel Milena Ouzunova Joachim Eder5 Birte Darnhofer5 Uschi Frei1 Yves Barrière6 Thomas Lubberstedt1 Abstract Background OMT O-methyltransferase genes are involved in lignin biosynthesis which relates to stover cell wall digestibility. Reduced lignin content is an important determinant of both forage quality and ethanol conversion efficiency of maize stover. Results Variation in genomic sequences coding for COMT CCoAOMTl and CCoAOMT2 was analyzed in relation to stover cell wall digestibility for a panel of 40 European forage maize inbred lines and re-analyzed for a panel of 34 lines from a published French study. Different methodologies for association analysis were performed and compared. Across association methodologies a total number of 25 12 1 6 COMT polymorphic sites were significantly associated with DNDF OMD NDF and WSC respectively. Association analysis for CCoAOMTl and CCoAOMT2 identified substantially fewer polymorphic sites 3 and 2 respectively associated with the investigated traits. Our re-analysis on the 34 lines from a published French dataset identified 14 polymorphic sites significantly associated with cell wall digestibility two of them were consistent with our study. Promising polymorphisms putatively causally associated with variability of cell wall digestibility were inferred from the total number of significantly associated SNPs Indels. Conclusions Several polymorphic sites for three O-methyltransferase loci were associated with stover cell wall digestibility. All three tested genes seem to be involved in controlling DNDF in particular COMT. Thus considerable variation among Bm3 wildtype alleles can be exploited