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Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes

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The two group I introns Nae.L1926 and Nmo.L2563, found at two different sites in nuclear LSU rRNA genes of Naegleria amoebo-flagellates, have been characterized in vitro. Their structural organization is related to that of the mobile Physarum intron Ppo.L1925 (PpLSU3) with ORFs extending the L1-loop of a typical group IC1 ribozyme. Nae.L1926, Nmo.L2563 and Ppo.L1925 RNAs all selfsplice in vitro, generating ligated exons and full-length intron circles as well as internal processed excised intron RNAs

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