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Báo cáo khoa học: " Organotypic slice culture of the hypothalamic paraventricular nucleus of rat"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Organotypic slice culture of the hypothalamic paraventricular nucleus of rat | J. Vet. Sci. 2007 8 1 15-20 JOURNAL OF Veterinary Science Organotypic slice culture of the hypothalamic paraventricular nucleus of rat Eun Seong Cho1 So Yeong Lee1 Jae-Yong Park2 Seong-Geun Hong2 Pan Dong Ryu1 1 Laboratory ofVeterinary Pharmacology BK21 Program for Veterinary Science and College ofVeterinary Medicine Seoul National University Seoul 151-742 Korea department ofPhysiology Institute ofHealth Science and Medical Research Center for Neural Dysfunction Gyeongsang National University College of Medicine Jinju 660-701 Korea Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus PVN of rat. The results showed that the slice cultures maintain the typical shape of the nucleus the immunocytochemical signals for oxytocin vasopressin and corticotropin-releasing hormone and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons and can be used in longterm studies on these neurons in vitro. Key words electrophysiology green fluorescent protein immunocytochemistry organotypic slice culture paraventricular nucleus Introduction Brain slices have been used as important tools for studying the physiological and morphological properties of the neuronal circuit as an in vitro model. In most studies experiments are performed using brain slices immediately after theft preparation. However the acute brain slice preparations have not been used in experiments over more than a day because the neuronal viability does not last for more than 12 h 6 . For this reason several .