Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa hoc:"Microsatellite loci in Japanese quail and cross-species amplification in chicken and guinea fowl"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Microsatellite loci in Japanese quail and cross-species amplification in chicken and guinea fowl | Genet. Sel. Evol. 34 2002 233-253 233 INRA EDP Sciences 2002 DOI 10.1051 gse 2002006 Original article Microsatellite loci in Japanese quail and cross-species amplification in chicken and guinea fowl Boniface Baboreka KAYANGa Miho lNOUE-MuRAYAMAb Takuya HoSHIb Koji Matsuo0 Hideaki Takahashic Mitsuru Minezawac Makoto MiZUTANid Shin ichi lTOb a The United Graduate School of Agricultural Science Gifu University Gifu 501-1193 Japan b Faculty of Agriculture Gifu University Gifu 501-1193 Japan c National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan d Laboratory Animal Research Station Nippon Institute for Biological Science Kobuchizawa 408-0041 Japan Received 28 June 2001 accepted 10 September 2001 Abstract - In line with the Gifu University s initiative to map the Japanese quail genome a total of 100 Japanese quail microsatellite markers isolated in our laboratory were evaluated in a population of 20 unrelated quails randomly sampled from a colony of wild quail origin. Ninety-eight markers were polymorphic with an average of 3.7 alleles per locus and a mean heterozygosity of 0.423. To determine the utility of these markers for comparative genome mapping in Phasianidae cross-species amplification of all the markers was tested with chicken and guinea fowl DNA. Amplification products similar in size to the orthologous loci in quail were observed in 42 loci in chicken and 20 loci in guinea fowl. Of the cross-reactive markers 57.1 in chicken and 55.0 in guineafowl were polymorphic when tested in 20 birds from their respective populations. Five of 15 markers that could cross-amplify Japanese quail chicken and guinea fowl DNA were polymorphic in all three species. Amplification of orthologous loci was confirmed by sequencing 10 loci each from chicken and guinea fowl and comparing with them the corresponding quail sequence. The microsatellite markers reported would serve as a useful resource base for genetic mapping in quail and comparative mapping in Phasianidae.