TheAspergillus nigerb-1,4-endogalactanase encoding gene (galA) was cloned and characterized. The expression ofgalA in A. nigerwas only detected in the presence of sugar beet pectin,D-galacturonic acid andL-arabinose, suggesting that galAis coregulatedwithboth thepectinolyticgenes aswell as the arabinanolytic genes. The corresponding enzyme, endogalactanase A (GALA), contains both active site resi-dues identified previously for thePseudomonas fluorescens b-1,4-endogalactanase.