Hesperis tosyaensis , a new species from northern Anatolia, is described and illustrated. It grows under open Quercus forest. The new species is distinct from the closely allied H. ozcelikii by the following features: stem erect, 45–90 cm tall, hispid, hairs 2– mm, and fruit glabrous. | Turkish Journal of Botany Research Article Turk J Bot (2016) 40: 87-96 © TÜBİTAK doi: New species and a synonym of the genus Hesperis (Brassicaceae) from Turkey Ahmet DURAN, Özlem ÇETİN* Department of Biology, Faculty of Science, Selçuk University, Selçuklu, Konya, Turkey Received: Accepted/Published Online: Final Version: Abstract: Hesperis tosyaensis , a new species from northern Anatolia, is described and illustrated. It grows under open Quercus forest. The new species is distinct from the closely allied H. ozcelikii by the following features: stem erect, 45–90 cm tall, hispid, hairs 2– mm, and fruit glabrous. Genetic differentiation between closely related Hesperis L. taxa and Matthiola was examined by molecular analyses of the ISSR. The chromosome number for Hesperis tosyaensis is 2n = 14. Information is also presented on its ecology and conservation status. In addition, Hesperis aspera . is treated as a synonym of Hesperis bicuspidata (Willd.) Poir. Key words: Hesperis, new species, ISSR, Cruciferae, systematics 1. Introduction Hesperis L. (Brassicaceae) is represented by many taxa at the junctions of the Irano-Turanian, Mediterranean, and Euro-Siberian phytogeographic regions; these regions meet in Anatolia. Towards the outer boundaries of each phytogeographic region, Hesperis is represented by fewer taxa, which is also indicated by the number of Hesperis taxa in various floras. There are 14 species in Europe, 11 species in Iran, 9 species in Romania, 5 species in Iraq, 3 species in Italy, and 1 species in Palestine (Săvulescu, 1955; Ball, 1964; Zohary, 1966; Dvořák, 1968, 1980; Pignatti, 1982; Duran and Hamzaoğlu, 2004; Duran and Ocak, 2005; Duran, 2005; Parolly and Tan, 2006; Duran, 2008, 2009). The genus Hesperis is represented by 31 species in the flora of Turkey (Cullen, 1965; Davis et al., 1988). Five new species have .