Hand hygiene is now regarded as one of the most important element of infection control activities. Correct steps of hand hygiene as stated by WHO using hand rubs was evaluated for all study subjects. Flora of hands was studied before and after hand rub by culture. The study was conducted in a tertiary care hospital attached to Karpagam Faculty of Medical Sciences and Research in Coimbatore, between June - August, 2016. A total of 40 subjects were included out of which there were doctors, medical students, nurses, hospital and sanitary attendants, who agreed to participate in the study and were on duty in hospital premises at the time of the study. | Efficiency of hand hygiene to reduce transient flora on the hands of health care workers in a tertiary care medical college hospital in Coimbatore, India