The aim of the research was to prepare collagen hydrolysate (CH) from unicorn leatherjacket (Aluterus monoceros) fish skin using papain at different hydrolysis temperature viz. 50C (CH-5), 200C (CH-20), 250C (CH-25) and 500C (CH-50) and to investigate the antioxidant properties (DPPH free radical-scavenging activity, ferric reducing power assay, ferric chelating activity and hydroxyl radical scavenging activity) prior to and after in vitro gastrointestinal (pepsin–pancreatin) simulated digestion in order to assess the bioaccessibility and biofunctional activities. The DH was higher () for CH-50 within 300 min of hydrolysis than other CH’s. On contrary, ferric reducing and ferric chelating properties were comparatively higher in CH-5 than other CH’s, corresponding to 33% and absorbance units, respectively at 8 mg/mL protein concentration. | Effect of hydrolysis temperature on in vitro bioaccessibility and antioxidant properties of unicorn leather jacket (Aluterus monoceros) skin collagen hydrolysates following simulated gastro-intestinal digestion
