Dextran can be produce by a wide range of gram positive and gram negative bacteria . Leuconostoc mesenteriods and Streptococcus mutant. In the present study dextran was produced from Leuconostoc mesenteriods. L. mesenteriods was isolated from idli batter identified by different biochemical parameters. Sucrose media was used for dextran production. Dextran was also produced using beet juice and compared with sucrose media. Optimum dextran production was considered according to the optimum pH, temperature and substrate concentration. The optimum pH ranged between and . | Production of dextran from Leuconostoc mesenteroid