Bài viết Định lượng đồng thời Kirenol, darutosid và darutigenol trong dược liệu Hy thiêm (Siegesbeckia orientalis L.) bằng HPLC-DPA tiến hành xây dựng phương pháp định lượng đồng thời kirenol, darutosid và darutigenol trong dược liệu hy thiêm sẽ góp phần làm cơ sở khoa học xây dựng tiêu chuẩn nguyên liệu đầu vào cho các sản phẩm thuốc và thực phẩm bảo vệ sức khỏe chứa hy thiêm. | VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 38 No. 2 2022 26-34 Original Article Simultaneous Quantification of Kirenol Daurutoside and Darutigenol in Siegesbeckia orientalis L. by HLPC-DPA Method Tran Thi Van Anh1 Pham Quoc Tuan1 Nguyen Van Khanh2 Dao Viet Hung1 Nguyen Thanh Hai2 Nguyen Quoc Tuan1 3 Nguyen Thi Minh Diep1 Ngo Thi Xuan Thinh1 Ha Thanh Hoa1 1 Phu Tho College of Medicine and Pharmacy 2201 Hung Vương Gia Cam Viet Tri Phu Tho Vietnam 2 VNU University of Medicine and Pharmacy 144 Xuan Thuy Cau Giay Hanoi Vietnam 3 College of Pharmacy Wonkwang University Iksan 54538 Korea Received 13 April 2022 Revised 24 May 2022 Accepted 25 May 2022 Abstract Kirenol 1 darutoside 2 and darutigenol 3 which are major active diterpenoids isolated from Herba Siegesbeckiae are reported for analgesic activity and inflammatory effects. Currently in Vietnamese Pharmacopoeia V there is no requirement for the contents of active ingredients in this medicinal plant. In this study a high-performance liquid chromatography HPLC method which was developed with good repeatability and recovery was used for simultaneous quantification of 1 2 and 3 in Herba Siegesbeckiae. This method was successfully applied to quantify 1-3 in samples of Herba Siegesbeckiae collected from different locations. The results showed that the content of compound 1 accounted for 0 mg g compound 2 ranged from to mg g and compound 3 ranged from to mg g. These results are considered a reference for upgrading the specification and quality control of Herba Siegesbeckiae. Keywords Herba Siegesbeckiae kirenol darutoside darutigenol HPLC. _ Corresponding author. E-mail address anhthu23081985@ https 2588-1132 26 T. T. V. Anh et al. VNU Journal of Science Medical and Pharmaceutical Sciences Vol. 38 No. 2 2022 26-34 27 Định lượng đồng thời Kirenol darutosid và darutigenol trong dược liệu Hy thiêm Siegesbeckia orientalis L. bằng HPLC-DPA Trần
