Bài viết Ảnh hưởng của NaOCl, môi trường và các chất điều hòa sinh trưởng đến nhân giống sắn KM140 sạch bệnh (Manihot esculenta Crantz) bằng phương pháp in vitro được nghiên cứu nhằm xác định nồng độ và thời gian khử trùng mẫu, đồng thời xác định môi trường nuôi cấy và nồng độ chất điều hòa sinh trưởng phù hợp cho việc nhân vô tính giống sắn KM140, giống sắn đang được trồng phổ biến tại khu vực phía Nam. | Trường Đại học Nông Lâm TP. Hồ Chí Minh 9 Effect of NaOCl growth media and plant growth regulators on in vitro propagation of disease-free KM140 cassava cultivar Manihot esculenta Crantz Duyen T. T. Nguyen1 Nien C. Nguyen1 Thanh T. Duong1 amp My T. Nguyen2 1 Crop Science Nong Lam University Ho Chi Minh City Vietnam 2 Department of Mushroom Research and Biotechnology Hung Loc Agricultural Experimental Research Center Dong Nai Vietnam ARTICLE INFO ABSTRACT Research Paper Cassava mosaic disease CMD is one of the most dangerous diseases that Received September 01 2021 has caused heavy losses in yield and starch content on cassava. In vitro Revised January 28 2022 propagation using disease-free cassava stakes was an optimal method to Accepted February 11 2022 produce healthy seedlings. In this study sodium hypochlorite NaOCl growth media MS MS and Knudson C and growth regulators BA Benzyl adenine NAA Naphthalene acetic acid GA Gibberellin were Keywords used to determine an appropriate time for sample sterilization and the suit- able concentration for shoot multiplication and root induction of KM140 cassava cultivar. The results showed that sample sterilization at 8 NaOCl Cassava concentration in 5 min gave the highest survival rate at 14 d of Growth media culture. Cassava explants cultured on MS medium supplemented with 1 Growth regulator mg L BA gained the highest number of shoots shoots shoot height In vitro propagation mm and the number of leaves leaves shoot at 50 d of cul- ture. The MS medium supplemented with mg L NAA and mg L Corresponding author GA was applicable for the rooting stage of KM140 cassava cultivar roots plantlet at 60 d of culture . The results of the study were funda- Nguyen Thi Thanh Duyen mental to in vitro propagation process of disease-free KM140 cassava mul- Email nt- tiplication. thanhduyen@ Cited as Nguyen D. T. T. Nguyen N. C. Duong T. T. amp Nguyen M. T. 2022 . Effect of NaOCl growth media and
