Nghiên cứu "Tái sinh cây đậu tương chuyển gene mang cấu trúc pZY102::GmDREB7A thông qua chọn lọc bằng phosphinothricin" nhằm tạo cây đậu tương chuyển gene phục vụ đánh giá vai trò của gene GmDREB7 đối với các gene liên quan đến tính chống chịu hạn và mặn của cây đậu tương. Các phương pháp biến nạp di truyền, tái sinh, chọn lọc in vitro và phân tích cây chuyển gene đã được sử dụng để tạo cây đậu tương chuyển gene phục vụ nghiên cứu chức năng của gene GmDREB7. | TNU Journal of Science and Technology 228 09 377 - 383 REGENERATION OF TRANSGENIC SOYBEAN PLANTS CARRYING THE STRUCTURE pZY102 GmDREB7A THROUGH SELECTION BY PHOSPHINOTHRICIN Nguyen Thi Hai Yen1 Duong Thu Ha2 Tran Thi Hong3 Nguyen Duc Hung3 Chu Hoang Mau3 1TNU - University of Sciences 2Bac Giang Department of Education and Training 3TNU - University of Education ARTICLE INFO ABSTRACT Received 14 5 2023 Soybean is a crop sensitive to drought and salinity stress so research on measures to improve drought and salinity tolerance of soybean is a matter Revised 08 6 2023 of current concern. The DREB protein is a transcription factor that Published 16 6 2023 enhances the expression of genes related to drought and salinity tolerance in soybean however there are many DREB genes in soybean whose function is still unknown. This study aimed to create transgenic soybean KEYWORDS plants to evaluate the role of the GmDREB7 gene on genes involved in the Genetic transformation abiotic stress tolerance of soybean. The methods of genetic transformation regeneration in vitro selection and analysis of transgenic plants were used GmDREB7A transgene to create transgenic soybean plants for the functional study of the Phosphinothricin GmDREB7 gene. From 150 samples transformed with Regeneration in vitro pZY102 GmDREB7A construct 447 shoots were obtained and 17 in vitro GmDREB7A transgenic plants were obtained after three screenings with Transgenic soybean phosphinothricin ppt . In vitro cloning and selection of T0 plants by ppt obtained 8 soybean lines denoted T0-1 T0-2 T0-3 T0-4 T0-5 T0-6 T07 T0- 8. Analysis of 8 lines of ppt-resistant soybeans in greenhouses by PCR resulted in 7 PCR-positive transgenic soybean lines T0-1 T0-2 T0-3 T0- 4 T0-5 T0-6 T07 T0-8 . The GmDREB7A transgenic soybean lines continued to be monitored and analyzed in subsequent generations. TÁI SINH CÂY ĐẬU TƯƠNG CHUYỂN GENE MANG CẤU TRÚC pZY102 GmDREB7A THÔNG QUA CHỌN LỌC BẰNG PHOSPHINOTHRICIN Nguyễn Thị Hải Yến1 .
