In this study, the antidengue virus activity of the ethanol extract from the leaves and twigs of E. cyathophora on four virus serotypes DENV1-DENV4 was examined. The result showed that the ethanol extract displayed antidengue activity on DENV1-4 with PRNT50 values of µg/mL. This extract was fractionated to the n-hexane and ethyl acetate fractions. | Cite this paper Vietnam J. Chem. 2023 61 3 371-377 Research article DOI Phytochemical study of Euphorbia cyathophora collected in Dan Phuong Hanoi and its antidengue activity against DENV1-4 virus serotypes Nguyen Thi Dung1 Pham Thi Ninh1 Nguyen Thi Luu1 Nguyen The Anh1 Vu Thi Bich Hau2 Nguyen Thi Thu Thuy2 Ho Ngoc Anh3 Phan Dieu Hang4 Tran Thi Phuong Thao1 5 1 Institute of Chemistry Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road Cau Giay Hanoi 10000 Viet Nam 2 National Institute of Hygiene and Epidemiology 1 Yersin Street Hai Ba Trung Hanoi 10000 Viet Nam 3 Institute of Biotechnology Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road Cau Giay Hanoi 10000 Viet Nam 4 Hanoi University of Medicine Nr. 1 Ton That Tung Street Kim Lien Dong Da Hanoi 10000 Viet Nam 5 Graduate University of Science and Technology Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road Cau Giay Hanoi 10000 Viet Nam Submitted March 30 2023 Revised May 17 2023 Accepted May 21 2023 Abstract The ethanol extract from the leaves and twigs of Euphorbia cyathophora showed antiviral activity against four dengue virus serotypes DENV1-4 with PRNT50 values of µg mL. Eight compounds including germanicol acetate 1 cymbopogone 2 1-heptacosanol 3 ethyl α-L-arabinofuranoside 4 quercetin 5 3-acetoxy-5 7 3 4 - tetrahydroxyflavone 6 3-acetoxy-5 7 4 -trihydroxyflavone 7 4 -acetoxy-3 5 7-trihydroxyflavone 8 were isolated from this extract. Compounds 2-8 were isolated for the first time from Euphorbia cyathophora. Antiviral activity evaluation of compounds 1-3 5-7 on DENV1-4 serotypes revealed that 7 was the most active compound with PRNT50 values of µM. Keywords. Euphorbia cyathophora antidengue activity secondary metabolites. 1. INTRODUCTION terpenoids. 2 4 6 7 In this study the antidengue virus activity of the ethanol extract from the leaves and Euphorbia cyathophora Vietnamese name Tieu twigs of E. cyathophora on four .
