The content of unsaturated fatty acids did differ between males and females of the two species studied. However, these differences are few and can be explained by the timing of their breeding seasons. The fatty acid composition of the fats from both investigated octopus species permits their use as food with nutritional value, and the production of bioactive additives. | Vietnam Journal of Marine Science and Technology 2023 23 3 303 310 Vietnam Academy of Science and Technology Vietnam Journal of Marine Science and Technology journal homepage jmst Composition and content of fatty acid lipid classes of two octopus species Amphioctopus neglectus and Cistopus indicus from Ha Long bay Quang Ninh province Vietnam Dinh Thi Ha1 Doan Lan Phuong2 Pham Van Chien1 Nguyen Van Quan1 Nguyen Duc The1 1 Institute of Marine Environment and Resources VAST Vietnam 2 Institute of Natural Products Chemistry VAST Vietnam Received 22 March 2023 Accepted 26 June 2023 ABSTRACT The fatty acid compositions of the lipids of two octopus species Amphiotopus neglectus and Cistopus indicus were investigated. The composition and content of fatty acids of two octopus samples were analyzed using a GC-MS. The percentages of the lipid classes in total lipid were determined based on the image analysis program Sorbfil TLC Videodensitometer DV Krasnodar Russia. The highest content of docosahexaenoic acid DHA from to of total fatty acids in the lipids of both species was revealed followed by the content of eicosapentaenoic acid EPA palmitic acid and arachidonic acid AA in the ranges and of total fatty acids respectively . The content of unsaturated fatty acids did differ between males and females of the two species studied. However these differences are few and can be explained by the timing of their breeding seasons. The fatty acid composition of the fats from both investigated octopus species permits their use as food with nutritional value and the production of bioactive additives. Keywords Fatty acid lipid class total lipid octopus Amphiotopus neglectus Cistopus indicus. Corresponding author at Institute of Marine Environment and Resources 246 Da Nang Ngo Quyen Hai Phong Vietnam. E-mail addresses ductheimer@ https 1859-3097 18184 ISSN 1859-3097 e-ISSN 2815-5904 2023 Vietnam .