Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Diversity of ectomycorrhizal symbionts in a disturbed Pinus halepensis plantation in the Mediterranean region. | Ann. For. Sci. 61 2004 705-710 INRA EDP Sciences 2004 DOI forest 2004069 705 Original article Diversity of ectomycorrhizal symbionts in a disturbed Pinus halepensis plantation in the Mediterranean region Khalid El KARKOURIa b Francis MARTINc Daniel MOUSAINa a UMR Sol et Environnement Équipe Rhizosphère et Symbiose 2 Place Viala 34060 Montpellier Cedex 1 France b UMR CNRS 6026 Interactions Cellulaires et Moléculaires Université de Rennes 1 Campus de Beaulieu bâtiment 13 35042 Rennes Cedex France c UMR INRA-UHP Interactions Arbres Micro-Organismes INRA-Nancy 54280 Champenoux France Received 10 September 2003 accepted 3 March 2004 Abstract - Ectomycorrhizal diversity ED associated with Pinus halepensis trees was examined years after outplanting at a fire-disturbed site of Rieucoulon Hérault France . ED analysis was examined on non-inoculated and Suillus collinitus-inoculated plants and on naturally regenerated trees. A total of 461 single ectomycorrhizas was typed using PCR-RFLP analysis and sequencing of the internal transcribed spacer ITS of the nuclear rDNA. Twelve ITS RFLP-taxa were detected. The ectomycorrhizal fungus S. collinitus ITS RFLP-taxon 1 was the most abundant species in the three treatments suggesting that it is a strong ectomycorrhizal competitor in this site. S. mediterraneensis ITS RFLP-taxon 2 was restricted to control and naturally regenerated trees and was unequally moderate . The remaining belowground ITS RFLP-taxa were uncommon and rare . The current experimental P. halepensis plantation showed a species-poor community dominated by two Suillus species. Ecological strategies of these symbionts are discussed. Pinus halepensis M. plantation ectomycorrhizal diversity PCR-RFLP-sequencing rDNA ITS Résumé - Diversité ectomycorhizienne dans une plantation à Pinus halepensis. La diversité génétique des ectomycorhizes de plants de P. halepensis a été examinée une année et demie après introduction dans .
