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Báo cáo hóa học: "Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients | Motta et al. Journal of Translational Medicine 2010 8 111 http www.translational-medicine.eom content 8 1 111 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients 1 2 2 f .-2 I 1 I z- -2 I-X -1 Marina Motta Marco Chiarini Claudia Ghidini Cinzia Zanotti Cinzia Lamorgese Luigi Caimi Giuseppe Rossi Luisa Imberti2 Abstract Background The immune defects occurring in chronic lymphocytic leukemia are responsible for the frequent occurrence of infections and autoimmune phenomena and may be involved in the initiation and maintenance of the malignant clone. Here we evaluated the quantitative defects of newly produced B and T lymphocytes. Methods The output of B and T lymphocytes from the production and maturation sites was analyzed in chronic lymphocytic leukemia patients and healthy controls by quantifying kappa-deleting recombination excision circles KRECs and T-cell receptor excision circles TRECs by a Real-Time PCR assay that simultaneously detects both targets. T-lymphocyte subsets were analyzed by six-color flow cytometric analysis. Data comparison was performed by two-sided Mann-Whitney test. Results KRECs level was reduced in untreated chronic lymphocytic leukemia patients studied at the very early stage of the disease whereas the release of TRECs cells was preserved. Furthermore the observed increase of CD4 lymphocytes could be ascribed to the accumulation of CD4 cells with effector memory phenotype. Conclusions The decreased number of newly produced B lymphocytes in these patients is likely related to a homeostatic mechanism by which the immune system balances the abnormal B-cell expansion. This feature may precede the profound defect of humoral immunity characterizing the later stages of the disease. Background Profound defects of both humoral and cell-mediated immunity have been described in patients with chronic lymphocytic leukemia CLL a disease .