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Báo cáo hóa học: " Bioinformatic analysis suggests that the Cypovirus 1 major core protein cistron harbours an overlapping gene"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Bioinformatic analysis suggests that the Cypovirus 1 major core protein cistron harbours an overlapping gene | Virology Journal BioMed Central Open Access Short report Bioinformatic analysis suggests that the Cypovirus I major core protein cistron harbours an overlapping gene Andrew E Firth and John F Atkins Address BioSciences Institute University College Cork Cork Ireland Email Andrew E Firth - A.Firth@ucc.ie John F Atkins - j.atkins@ucc.ie Corresponding author Published 20 May 2008 Received 16 April 2008 Accepted 20 May 2008 Virology journal 2008 5 62 doi 10.1186 1743-422X-5-62 This article is available from http www.virologyj.cOm content 5 1 62 2008 Firth and Atkins licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Members of the genus Cypovirus family Reoviridae are common pathogens of insects. These viruses have linear dsRNA genomes divided into 10-11 segments which have generally been assumed to be monocistronic. Here bioinformatic evidence is presented for a short overlapping coding sequence CDS in the cypovirus genome segment encoding the major core capsid protein VP1 overlapping the 5 -terminal region of the VP1 ORF in the 1 reading frame. In Cypovirus type 1 CPV-1 a 62-codon AUG-initiated open reading frame hereafter ORFX is present in all four available segment 1 sequences. The pattern of base variations across the sequence alignment indicates that ORFX is subject to functional constraints at the amino acid level even when the constraints due to coding in the overlapping VP1 reading frame are taken into account MLOGD software . In fact the translated ORFX shows greater amino acid conservation than the overlapping region of VP1. The genomic location of ORFX is consistent with translation via leaky scanning. A 62-64 codon AUG-initiated ORF is present in a corresponding location and reading frame in other available .