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Báo cáo hóa học: " Neuraminidase activity provides a practical read-out for a high throughput influenza antiviral screening assay"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Neuraminidase activity provides a practical read-out for a high throughput influenza antiviral screening assay | Virology Journal BioMed Central Open Access Methodology Neuraminidase activity provides a practical read-out for a high throughput influenza antiviral screening assay Maryna C Eichelberger 1 3 Arash Hassantoufighi1 Meng Wu2 and Min Li2 Address 1Center for Biologics Evaluation and Research Food and Drug Administration Bethesda MD USA 2High Throughput Biology Center and Department of Neuroscience Johns Hopkins School of Medicine Baltimore MD USA and 3Division of Viral Products OVRR CBER FDA 8800 Rockville Pike Building 29A 1D24 Bethesda MD 20892 USA Email Maryna C Eichelberger - Maryna.Eichelberger@fda.hhs.gov Arash Hassantoufighi - Arash.Hassantoufighi@fda.hhs.gov Meng Wu - meng@jhmi.edu Min Li - minli@jhmi.edu Corresponding author Published 26 September 2008 Virology Journal 2008 5 109 doi l0.ll86 l743-422X-5-l09 Received 9 September 2008 Accepted 26 September 2008 This article is available from http www.virologyj.cOm content 5 l l09 2008 Eichelberger et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The emergence of influenza strains that are resistant to commonly used antivirals has highlighted the need to develop new compounds that target viral gene products or host mechanisms that are essential for effective virus replication. Existing assays to identify potential antiviral compounds often use high throughput screening assays that target specific viral replication steps. To broaden the search for antivirals cell-based replication assays can be performed but these are often labor intensive and have limited throughput. Results We have adapted a traditional virus neutralization assay to develop a practical cell-based