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Báo cáo hóa học: " A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene | Virology Journal BioMed Central Methodology Open Access A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene Larry A Hanson 1 Mary R Rudis1 Marcia Vasquez-Lee1 and Roy D Montgomery2 Address Department of Basic Sciences College of Veterinary Medicine Mississippi State University P.O. Box 6100 Mississippi State Mississippi 39762 USA and 2Department of Pathobiology and Population Medicine College of Veterinary Medicine Mississippi State University P.O. Box 6100 Mississippi State Mississippi 39762 USA Email Larry A Hanson - hanson@cvm.msstate.edu Mary R Rudis - mrudis@utk.edu Marcia Vasquez-Lee - mvlee@cvm.msstate.edu Roy D Montgomery - montgomery@cvm.msstate.edu Corresponding author Published II April 2006 Received 16 November 2005 Accepted 1 1 April 2006 VirologyJournal2006 3 28 doi 10.1186 1 743-422X-3-28 This article is available from http www.virologyj.cOm content 3 1 28 2006Hanson et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Many viral pathogens are poorly characterized are difficult to culture or reagents are lacking for confirmatory diagnoses. We have developed and tested a robust assay for detecting and characterizing large DNA viruses and adenoviruses. The assay is based on the use of degenerate PCR to target a gene common to these viruses the DNA polymerase and sequencing the products. Results We evaluated our method by applying it to fowl adenovirus isolates catfish herpesvirus isolates and largemouth bass ranavirus iridovirus from cell culture and lymphocystis disease virus iridovirus and avian poxvirus from tissue. All viruses with the exception of avian poxvirus produced the expected product. After optimization of extraction .