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báo cáo hóa học:" Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma | Virology Journal BioMed Central Research Open Access Synergistic inhibition of human cytomegalovirus replication by interferon-alpha beta and interferon-gamma Bruno Sainz Jr Heather L LaMarcah Robert F Garry and Cindy A Morris Address Department of Microbiology and Immunology Program in Molecular Pathogenesis and Immunity Tulane University Health Sciences Center 1430 Tulane Avenue SL-38 New Orleans LA 70112 USA Email Bruno Sainz - bsainz@scripps.edu Heather L LaMarca - hlamarc@tulane.edu Robert F Garry - rfgarry@tulane.edu Cindy A Morris - cmorris2@tulane.edu Corresponding author tEqual contributors Published 23 February 2005 Received 17 February 2005 Accepted 23 February 2005 Virology Journal 2005 2 14 doi 10.1186 1743-422X-2-14 This article is available from http www.virologyj.cOm content 2 1 14 2005 Sainz et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Recent studies have shown that gamma interferon IFN-y synergizes with the innate IFNs IFN-a and IFN-P to inhibit herpes simplex virus type 1 HSV-1 replication in vitro. To determine whether this phenomenon is shared by other herpesviruses we investigated the effects of IFNs on human cytomegalovirus HCMV replication. Results We have found that as with HSV-1 IFN-y synergizes with the innate IFNs IFN-a p to potently inhibit HCMV replication in vitro. While pre-treatment of human foreskin fibroblasts HFFs with IFN-a IFN-P or IFN-y alone inhibited HCMV plaque formation by 30 to 40-fold treatment with IFN-a and IFN-y or IFN-P and IFN-y inhibited HCMV plaque formation by 163- and 662-fold respectively. The generation of isobole plots verified that the observed inhibition of HCMV plaque formation and replication in HFFs by IFN-a p and IFN-y was a .