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Báo cáo hóa học: " Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Cloning and overexpression of a new chitosanase gene from Penicillium sp. D- | AMB Express SpringerOpen0 This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text HTML versions will be made available soon. Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1 AMB Express 2012 2 13 doi 10.1186 2191-0855-2-13 Xu-Fen Zhu xufenzhu@zju.edu.cn Hai-Qin Tan selery2009@yahoo.cn Chu Zhu zucu@qq.com Li Liao liaoli1115@163.com Xin-Qi Zhang meanow@126.com Min Wu wumin@zju.edu.cn ISSN 2191-0855 Article type Original Submission date 21 September 2011 Acceptance date 16 February 2012 Publication date 16 February 2012 Article URL http www.amb-express.com content 2 1 13 This peer-reviewed article was published immediately upon acceptance. It can be downloaded printed and distributed freely for any purposes see copyright notice below . Articles in AMB Express are listed in PubMed and archived at PubMed Central. For information about publishing your research in AMB Express go to http www.amb-express.com authors instructions For information about other SpringerOpen publications go to http www.springeropen.com 2012 Zhu et al. licensee Springer. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Cloning and overexpression of a new chitosanase gene from Penỉcỉllỉum sp. D-1 Xu-Fen Zhu Hai-Qin Tan Chu Zhu Li Liao Xin-Qi Zhang and Min Wu College of Life Sciences Zhejiang University Hangzhou 310058 P R. China Correspondence Xu-Fen Zhu xufenzhu@zju.edu.cn Tel 86-571-88206595 HQT selery2009@yahoo.cn CZ zucu@qq.com LL liaoli1115@163.com XQZ meanow@126.com MW wumin@zju.edu.cn Abstract A chitosanase gene csn was cloned from Penicillium sp. D-1 by inverse PCR. The cDNA sequence analysis revealed that csn had no intron. The deduced CSN protein consists of 250 amino acids including a .