Exploiting specific targets is of specific interest in developing eco-friendly pesticides. We isolated, purified and characterized a novel b-N-acetyl-d-hexosaminidase (OfHex1) from the fifth instar larva integument of the Asian corn borer,Ostrinia furnacalis(Guene´e). OfHex1 was purified 1468-fold to homogeneity with an activity yield of 20% by four column chroma-tography steps.